Author: Usui, Kimihito; Ichihashi, Norikazu; Yomo, Tetsuya
Title: A design principle for a single-stranded RNA genome that replicates with less double-strand formation Document date: 2015_9_18
ID: znhvdtg8_11
Snippet: RNAs encoding the â£-domain gene were mixed with the highly purified reconstituted translation system (30) containing protein (EA reagent, Clontech) and a fluorescent substrate (50 M Tokyo Green R -â¤Gal, Sekisui Medical, Japan) according to a previous study (31) . The fluorescence at 37 • C was detected using an Mx3005P QPCR system (Agilent Technologies, USA)......
Document: RNAs encoding the â£-domain gene were mixed with the highly purified reconstituted translation system (30) containing protein (EA reagent, Clontech) and a fluorescent substrate (50 M Tokyo Green R -â¤Gal, Sekisui Medical, Japan) according to a previous study (31) . The fluorescence at 37 • C was detected using an Mx3005P QPCR system (Agilent Technologies, USA).
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