Selected article for: "previous study and RNA synthesis"

Author: Usui, Kimihito; Ichihashi, Norikazu; Yomo, Tetsuya
Title: A design principle for a single-stranded RNA genome that replicates with less double-strand formation
  • Document date: 2015_9_18
  • ID: znhvdtg8_22
    Snippet: We next investigated the effect of insertion of sequences that form stem structures. We performed replication experiments with RNAs inserted with 20-to 80-nt stem structures consisting of G and A on one side and C and U on the other side of a stem structure (13. Stem GA20, 14. Stem GA40 and 15. Stem GA80) and measured the total RNA synthesis and the dsRNA ratios as described above. Both the total RNA and the dsRNA ratios exhibited a tendency to d.....
    Document: We next investigated the effect of insertion of sequences that form stem structures. We performed replication experiments with RNAs inserted with 20-to 80-nt stem structures consisting of G and A on one side and C and U on the other side of a stem structure (13. Stem GA20, 14. Stem GA40 and 15. Stem GA80) and measured the total RNA synthesis and the dsRNA ratios as described above. Both the total RNA and the dsRNA ratios exhibited a tendency to decrease slightly as the stem size increased ( Figure 4A and B), consistent with a previous study (26) and our model Supplementary Table S1. shown in Figure 1 , which predicts that the insertion of stem structures does not facilitate dsRNA formation.

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