Title: 2017 ACVIM Forum Research Abstract Program Document date: 2017_6_15
ID: ri2w5iby_761
Snippet: Metronidazole (15 mg/kg po q12 h) was administered to adult horses (n = 5) with indwelling cecal cannulas (AUP 2013-0123). Fecal and cecal samples were taken on days À52, À28, À14, 0, 3 (fecal only), 7, and 14. Illumina sequencing of 16S rRNA genes was performed on extracted DNA. Data were analyzed using Quantitative Insights Into Molecular Ecology (QIIME) and analysis of similarities (ANOSIM) was performed. Univariate analysis of bacterial gr.....
Document: Metronidazole (15 mg/kg po q12 h) was administered to adult horses (n = 5) with indwelling cecal cannulas (AUP 2013-0123). Fecal and cecal samples were taken on days À52, À28, À14, 0, 3 (fecal only), 7, and 14. Illumina sequencing of 16S rRNA genes was performed on extracted DNA. Data were analyzed using Quantitative Insights Into Molecular Ecology (QIIME) and analysis of similarities (ANOSIM) was performed. Univariate analysis of bacterial groups was performed using a Friedman's test and Dunn's post-test. Linear discriminant analysis effects size (LEfSe) was used to find bacterial taxa associated with study day. Short chain fatty acids (SCFA) and untargeted metabolomics were analyzed using mass spectrometry platforms.
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