Selected article for: "RNA polymerase and Supplementary table"
Author: Usui, Kimihito; Ichihashi, Norikazu; Yomo, Tetsuya
Title: A design principle for a single-stranded RNA genome that replicates with less double-strand formation
  • Document date: 2015_9_18
    • ID: znhvdtg8_6
    Snippet: The RNAs with 28 insertions shown in Figure 2 were constructed by PCR amplification of a plasmid encoding the MDV-1 sequence (pUC-MDV-LR (27) ) with primers that consist of a common region and an insertion sequence attached to the 5 -end (sequences of the insertions are shown in Supplementary Table S1 ). The common regions of the primers are ACGGGCTAGCGCTTTC and CGTCACG-GTCGAACTCCC. The inserted sequences are shown in Supplementary Table S1 . Eac.....
    Document: The RNAs with 28 insertions shown in Figure 2 were constructed by PCR amplification of a plasmid encoding the MDV-1 sequence (pUC-MDV-LR (27) ) with primers that consist of a common region and an insertion sequence attached to the 5 -end (sequences of the insertions are shown in Supplementary Table S1 ). The common regions of the primers are ACGGGCTAGCGCTTTC and CGTCACG-GTCGAACTCCC. The inserted sequences are shown in Supplementary Table S1 . Each RNA was synthesized by in vitro transcription with T7 RNA polymerase (Takara, Japan) after SmaI (Toyobo, Osaka, Japan) digestion to determine the 3 end. The synthesized RNA was purified using a column (RNeasy mini, QIAGEN) before and after digestion of the remaining template DNA with DNase I (Takara, Japan).

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