Title: A Golgi retention signal in a membrane-spanning domain of coronavirus E1 protein Document date: 1991_10_1
ID: s4a8zs5a_26
Snippet: Gml was not transported to the plasma membrane, however. Indirect immunofluorescence microscopy demonstrated that Gml was absent from the cell surface but present in a juxtanuclear region consistent with Golgi localization (Fig . 2 ) . In addition, the two N-linked oligosaccharides added to Gml were not processed to an endo H-resistant form as they 23 were on wild-type G protein. After a 10 min pulse label, wild-type G protein became endo H resi.....
Document: Gml was not transported to the plasma membrane, however. Indirect immunofluorescence microscopy demonstrated that Gml was absent from the cell surface but present in a juxtanuclear region consistent with Golgi localization (Fig . 2 ) . In addition, the two N-linked oligosaccharides added to Gml were not processed to an endo H-resistant form as they 23 were on wild-type G protein. After a 10 min pulse label, wild-type G protein became endo H resistant with a halftime of about 20 min, whereas Gml was endo H sensitive even after 4 h of chase (see Fig. 10 ). This suggested that the Gml protein was retained in a pre-medial Golgi compartment, like the wild-type El protein .
Search related documents:
Co phrase search for related documents- Golgi localization and indirect immunofluorescence microscopy: 1, 2, 3, 4, 5
- Golgi localization and juxtanuclear region: 1
- Golgi localization and plasma membrane: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24
- Golgi localization and plasma membrane transport: 1, 2
- Golgi localization and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
- immunofluorescence microscopy and indirect immunofluorescence microscopy: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- immunofluorescence microscopy and plasma membrane: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28
- immunofluorescence microscopy and plasma membrane transport: 1, 2, 3, 4
- immunofluorescence microscopy and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26
- immunofluorescence microscopy and wild type El protein: 1
- indirect immunofluorescence microscopy and plasma membrane: 1, 2, 3, 4, 5
- indirect immunofluorescence microscopy and plasma membrane transport: 1, 2
- indirect immunofluorescence microscopy and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- indirect immunofluorescence microscopy and wild type El protein: 1
- juxtanuclear region and plasma membrane: 1, 2
- juxtanuclear region and plasma membrane transport: 1
- juxtanuclear region and wild type: 1
- plasma membrane and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64
- plasma membrane transport and wild type: 1, 2, 3, 4, 5, 6
Co phrase search for related documents, hyperlinks ordered by date