Title: A Golgi retention signal in a membrane-spanning domain of coronavirus E1 protein Document date: 1991_10_1
ID: s4a8zs5a_26
Snippet: Gml was not transported to the plasma membrane, however. Indirect immunofluorescence microscopy demonstrated that Gml was absent from the cell surface but present in a juxtanuclear region consistent with Golgi localization (Fig . 2 ) . In addition, the two N-linked oligosaccharides added to Gml were not processed to an endo H-resistant form as they 23 were on wild-type G protein. After a 10 min pulse label, wild-type G protein became endo H resi.....
Document: Gml was not transported to the plasma membrane, however. Indirect immunofluorescence microscopy demonstrated that Gml was absent from the cell surface but present in a juxtanuclear region consistent with Golgi localization (Fig . 2 ) . In addition, the two N-linked oligosaccharides added to Gml were not processed to an endo H-resistant form as they 23 were on wild-type G protein. After a 10 min pulse label, wild-type G protein became endo H resistant with a halftime of about 20 min, whereas Gml was endo H sensitive even after 4 h of chase (see Fig. 10 ). This suggested that the Gml protein was retained in a pre-medial Golgi compartment, like the wild-type El protein .
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