Title: A Golgi retention signal in a membrane-spanning domain of coronavirus E1 protein Document date: 1991_10_1
ID: s4a8zs5a_26
Snippet: Gml was not transported to the plasma membrane, however. Indirect immunofluorescence microscopy demonstrated that Gml was absent from the cell surface but present in a juxtanuclear region consistent with Golgi localization (Fig . 2 ) . In addition, the two N-linked oligosaccharides added to Gml were not processed to an endo H-resistant form as they 23 were on wild-type G protein. After a 10 min pulse label, wild-type G protein became endo H resi.....
Document: Gml was not transported to the plasma membrane, however. Indirect immunofluorescence microscopy demonstrated that Gml was absent from the cell surface but present in a juxtanuclear region consistent with Golgi localization (Fig . 2 ) . In addition, the two N-linked oligosaccharides added to Gml were not processed to an endo H-resistant form as they 23 were on wild-type G protein. After a 10 min pulse label, wild-type G protein became endo H resistant with a halftime of about 20 min, whereas Gml was endo H sensitive even after 4 h of chase (see Fig. 10 ). This suggested that the Gml protein was retained in a pre-medial Golgi compartment, like the wild-type El protein .
Search related documents:
Co phrase search for related documents- cell surface and Gml protein: 1, 2, 3, 4
- cell surface and Golgi compartment: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- cell surface and Golgi localization: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
- cell surface and immunofluorescence microscopy: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- cell surface and indirect immunofluorescence microscopy: 1, 2, 3, 4, 5
- cell surface and juxtanuclear region: 1
- cell surface and plasma membrane: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- cell surface and plasma membrane transport: 1, 2, 3, 4, 5, 6, 7, 8, 9
- El protein and Gml protein: 1, 2
- El protein and Golgi compartment: 1, 2
- El protein and Golgi localization: 1, 2
- El protein and immunofluorescence microscopy: 1, 2, 3, 4
- El protein and indirect immunofluorescence microscopy: 1, 2, 3, 4
- El protein and plasma membrane: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- El protein and plasma membrane transport: 1, 2, 3
- Gml protein and Golgi compartment: 1
- Gml protein and Golgi localization: 1
- Gml protein and plasma membrane: 1, 2, 3, 4, 5, 6
- Golgi compartment and plasma membrane transport: 1, 2, 3, 4
Co phrase search for related documents, hyperlinks ordered by date