Author: Liu, Xingjian; Yang, Xin; Mehboob, Arslan; Hu, Xiaoyuan; Yi, Yongzhu; Li, Yinü; Zhang, Zhifang
Title: A construction strategy for a baculovirus-silkworm multigene expression system and its application for coexpression of type I and type II interferons Document date: 2019_12_19
ID: uxzeg16r_13
Snippet: Fifth instar silkworm larvae or pupae were injected with 5 μl of cell culture media containing recombinant BmNPV (10 7 -10 8 plaqueforming units (PFU) mL −1 ) between abdominal knobs on the backside. Silkworm larvae and pupae were reared at 25 ~ 27°C and 65% humidity for 108-120 hr. Larval hemolymph was collected by cutting prolegs, and 1-phenyl-2-thiourea was added at 0.1 mM to prevent melanization. Larval hemolymph and pupae were stored at .....
Document: Fifth instar silkworm larvae or pupae were injected with 5 μl of cell culture media containing recombinant BmNPV (10 7 -10 8 plaqueforming units (PFU) mL −1 ) between abdominal knobs on the backside. Silkworm larvae and pupae were reared at 25 ~ 27°C and 65% humidity for 108-120 hr. Larval hemolymph was collected by cutting prolegs, and 1-phenyl-2-thiourea was added at 0.1 mM to prevent melanization. Larval hemolymph and pupae were stored at −20°C for subsequent assays.
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