Selected article for: "cell density and milliliter cell"
Author: Liu, Xingjian; Yang, Xin; Mehboob, Arslan; Hu, Xiaoyuan; Yi, Yongzhu; Li, Yinü; Zhang, Zhifang
Title: A construction strategy for a baculovirus-silkworm multigene expression system and its application for coexpression of type I and type II interferons
  • Document date: 2019_12_19
    • ID: uxzeg16r_15
    Snippet: According to published procedures (Rein & Rubin, 1968) , chicken embryo fibroblasts (CEFs) were prepared. A CEF cell suspension was isolated from 9-to 11-day-old chicken embryos. The cell concentration was approximately 1 × 10 6 cells per milliliter. CEF cells were seeded in 96-well plates at a constant cell density of 1 × 10 5 cells per well for 8-12 hr at 37°C in 5% CO 2 in air......
    Document: According to published procedures (Rein & Rubin, 1968) , chicken embryo fibroblasts (CEFs) were prepared. A CEF cell suspension was isolated from 9-to 11-day-old chicken embryos. The cell concentration was approximately 1 × 10 6 cells per milliliter. CEF cells were seeded in 96-well plates at a constant cell density of 1 × 10 5 cells per well for 8-12 hr at 37°C in 5% CO 2 in air.

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