A Golgi retention signal in a membrane-spanning domain of coronavirus E1 protein

It should be noted that the only protein whose localization we have determined at the electron microscope level is the wild-type El protein . Although both the immunofluores- Figure 6 . Indirect immunofluorescence microscopy of mutant El proteins . Transfected COS cells were fixed, permeabilized, and stained for El using the anti-peptide antibody which recognizes the El cytoplasmic tail, followed by a Texas red-conjugated second antibody. With the exception of LQ3o, all of the mutation appeared to hinder exit of the mutant proteins from the ER . Bar, 10 jm . cence data and the endo H-sensitive oligosaccharides on Om2,3 and Gml are consistent with cis-Golgi localization, absolute verification awaits immunoelectron microscopy.