Selected article for: "dsRNA formation and formation model"
Author: Usui, Kimihito; Ichihashi, Norikazu; Yomo, Tetsuya
Title: A design principle for a single-stranded RNA genome that replicates with less double-strand formation
  • Document date: 2015_9_18
    • ID: znhvdtg8_4
    Snippet: In this study, we attempted to determine a simple principle to help design an ssRNA that encodes a gene and that continuously replicates without dsRNA formation using Q␤ replicase. We first propose a structure-dependent dsRNA formation model and then examine the model by measuring replication of RNAs with various structures. From the results, we extracted a simple rule to design an ss-RNA that replicates with less dsRNA formation and actu-ally .....
    Document: In this study, we attempted to determine a simple principle to help design an ssRNA that encodes a gene and that continuously replicates without dsRNA formation using Q␤ replicase. We first propose a structure-dependent dsRNA formation model and then examine the model by measuring replication of RNAs with various structures. From the results, we extracted a simple rule to design an ss-RNA that replicates with less dsRNA formation and actu-ally designed a new replicable RNA that encodes a domain of the ␤-galactosidase gene.

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