Author: Liu, Xingjian; Yang, Xin; Mehboob, Arslan; Hu, Xiaoyuan; Yi, Yongzhu; Li, Yinü; Zhang, Zhifang
Title: A construction strategy for a baculovirus-silkworm multigene expression system and its application for coexpression of type I and type II interferons Document date: 2019_12_19
ID: uxzeg16r_16
Snippet: A GFP-reduction assay with VSV-GFP was used to assay the antiviral activity of recombinant chicken IFNs in CEFs. Larval hemolymph containing IFN expression product was treated by ultrasonication. After centrifugation, the supernatant of the IFN product was filtered with a 0.22 μm syringe filter. Interferon supernatant was gradient diluted in DMEM with 7% FBS. After incubation with diluted IFN for 18-24 hr at 37°C in 5% CO 2 in air, CEF cells we.....
Document: A GFP-reduction assay with VSV-GFP was used to assay the antiviral activity of recombinant chicken IFNs in CEFs. Larval hemolymph containing IFN expression product was treated by ultrasonication. After centrifugation, the supernatant of the IFN product was filtered with a 0.22 μm syringe filter. Interferon supernatant was gradient diluted in DMEM with 7% FBS. After incubation with diluted IFN for 18-24 hr at 37°C in 5% CO 2 in air, CEF cells were infected with VSV-GFP at 10 TCID 50 per well. After 24 hr, cell cytopathic effect (CPE) and green fluorescence were detected. The antiviral activity of IFNs was calculated according to the CPE reduction method (Ge et al., 2006) .
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