Selected article for: "antiviral activity and coexpression product antiviral activity"
Author: Liu, Xingjian; Yang, Xin; Mehboob, Arslan; Hu, Xiaoyuan; Yi, Yongzhu; Li, Yinü; Zhang, Zhifang
Title: A construction strategy for a baculovirus-silkworm multigene expression system and its application for coexpression of type I and type II interferons
  • Document date: 2019_12_19
    • ID: uxzeg16r_18
    Snippet: IFN-α is both acid and heat resistant. However, IFN-γ is easily deactivated by both acid (pH 2.0) and temperature (56°C) (Ho, Armstrong, & Breinig, 1975) . Thus, the antiviral activity of IFN-α in the coexpression product was studied. The pH value of larval hemolymph or pupa product was adjusted to 2.0 ± 0.2 using a hydrochloric acid solution (0.1 M). After 24 hr of static incubation at 4°C and filtration, the pH value was adjusted to 7.0 Â.....
    Document: IFN-α is both acid and heat resistant. However, IFN-γ is easily deactivated by both acid (pH 2.0) and temperature (56°C) (Ho, Armstrong, & Breinig, 1975) . Thus, the antiviral activity of IFN-α in the coexpression product was studied. The pH value of larval hemolymph or pupa product was adjusted to 2.0 ± 0.2 using a hydrochloric acid solution (0.1 M). After 24 hr of static incubation at 4°C and filtration, the pH value was adjusted to 7.0 ± 0.1 using a sodium hydroxide solution (1 M). After 1 hr of static incubation at 4°C and filtration, the sample was heat treated (56°C) for 30 min. After filtration with a 0.22 μm syringe filter, IFN-γ of the product was deactivated.

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