Author: Liu, Xingjian; Yang, Xin; Mehboob, Arslan; Hu, Xiaoyuan; Yi, Yongzhu; Li, Yinü; Zhang, Zhifang
Title: A construction strategy for a baculovirus-silkworm multigene expression system and its application for coexpression of type I and type II interferons Document date: 2019_12_19
ID: uxzeg16r_2
Snippet: Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the most widely employed baculoviruses for gene expression. In the last 3 decades, BmNPV has undergone modifications in many ways to generate recombinant BmNPV that is more convenient and enhances the expression efficiency of foreign genes (Kato, Kajikawa, Maenaka, & Park, 2010; Maeda et al., 1985) . Compared with the AcMNPV-cell expression system, the BmNPV-silkworm system possesses better postt.....
Document: Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the most widely employed baculoviruses for gene expression. In the last 3 decades, BmNPV has undergone modifications in many ways to generate recombinant BmNPV that is more convenient and enhances the expression efficiency of foreign genes (Kato, Kajikawa, Maenaka, & Park, 2010; Maeda et al., 1985) . Compared with the AcMNPV-cell expression system, the BmNPV-silkworm system possesses better posttranslational processing and greater expression efficiency (Dojima et al., 2009; Usami et al., 2011 ). Previously, we successfully constructed a reBmBac system for recombinant BmNPV with increased efficiency (Liu et al., 2016) . By utilizing this system, researchers can proficiently and rapidly obtain recombinant baculoviruses and target proteins in silkworms.
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