Selected article for: "dsRNA ratio and RNA synthesis"
Author: Usui, Kimihito; Ichihashi, Norikazu; Yomo, Tetsuya
Title: A design principle for a single-stranded RNA genome that replicates with less double-strand formation
  • Document date: 2015_9_18
    • ID: znhvdtg8_23
    Snippet: We investigated the effect of the stability of stem structures by changing the GC ratio of the inserted stem structures. We performed replication experiments with RNAs inserted with 40-nt stems with 25-75% GC ratios (14. Stem GA40, 16. Stem GAAA40, 17. Stem GAA40, 18. Stem GGGA40 and 19. Stem GGA40) as described above. Both the total RNA synthesis ( Figure 4C ) and the dsRNA ratios ( Figure 4D ) did not change significantly in this range of GC ra.....
    Document: We investigated the effect of the stability of stem structures by changing the GC ratio of the inserted stem structures. We performed replication experiments with RNAs inserted with 40-nt stems with 25-75% GC ratios (14. Stem GA40, 16. Stem GAAA40, 17. Stem GAA40, 18. Stem GGGA40 and 19. Stem GGA40) as described above. Both the total RNA synthesis ( Figure 4C ) and the dsRNA ratios ( Figure 4D ) did not change significantly in this range of GC ratios, indicating that the stability of a 40-nt stem with a 25% GC ratio is sufficient to inhibit dsRNA formation.

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