Selected article for: "µg ml and fetal bovine serum"
Author: Chen, Yongxiong; Chan, Vera Sau-Fong; Zheng, Bojian; Chan, Kelvin Yuen-Kwong; Xu, Xiaoning; To, Leo Yuk-Fai; Huang, Fang-Ping; Khoo, Ui-Soon; Lin, Chen-Lung Steve
Title: A novel subset of putative stem/progenitor CD34(+)Oct-4(+) cells is the major target for SARS coronavirus in human lung
  • Document date: 2007_10_29
    • ID: u4qhk802_27
    Snippet: Lung tissues were excised from lung cancer patients for ex vivo infection with the approval by the Institutional Review Board of the University of Hong Kong. Fresh lung tissues were cut into small tissue blocks (2 -3 mm 3 ), followed by SARS-CoV (strain GZ50) ( 33 ) infection at 5 ϫ 10 6 TCID 50 in 1 ml of serum-free RPMI medium at 37 ° C for 1 h. The culture was then supplemented with 10% fetal bovine serum and further incubated at 37 ° C for.....
    Document: Lung tissues were excised from lung cancer patients for ex vivo infection with the approval by the Institutional Review Board of the University of Hong Kong. Fresh lung tissues were cut into small tissue blocks (2 -3 mm 3 ), followed by SARS-CoV (strain GZ50) ( 33 ) infection at 5 ϫ 10 6 TCID 50 in 1 ml of serum-free RPMI medium at 37 ° C for 1 h. The culture was then supplemented with 10% fetal bovine serum and further incubated at 37 ° C for 16 h. Tissue blocks were fi xed and processed for subsequent IHC, FISH, and ISH analysis as described above.

    Search related documents:
    Co phrase search for related documents
    • cancer patient and Lung tissue: 1, 2
    • ex vivo infection and Lung tissue: 1, 2
    • fetal bovine and Lung tissue: 1
    • fetal bovine and RPMI medium: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • fetal bovine and serum free RPMI medium: 1, 2, 3, 4
    • fetal bovine serum and Lung tissue: 1
    • fetal bovine serum and RPMI medium: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • fetal bovine serum and serum free RPMI medium: 1, 2, 3, 4
    • fresh lung tissue and Lung tissue: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • Lung tissue and tissue block: 1
    • RPMI medium and serum free RPMI medium: 1, 2, 3, 4, 5