Author: Liu, Chunxi; Liu, Tingxian; Yu, Xiaoyue; Gu, Yizhu
Title: A preliminary study on the interaction between Asn-Gly-Arg (NGR)-modified multifunctional nanoparticles and vascular epithelial cells Document date: 2017_4_1
ID: xio0g203_12
Snippet: To observe the localization of CD13, we treated HUVEC cultured in 35-mm glass bottom dishes (Corning Incorporated, USA) with PE-conjugated anti-CD13 antibody (1:100 dilution) for 3 h at 4 1C, rinsed the cells, and incubated them for 0-3 h at 37 1C. After being fixed with 4% paraformaldehyde in 0.1 mol/L PBS for 15 min, the cells were permeabilized with 0.1% Triton X-100 for 5 min, treated with 3% bovine serum albumin for 20 min, and labelled for .....
Document: To observe the localization of CD13, we treated HUVEC cultured in 35-mm glass bottom dishes (Corning Incorporated, USA) with PE-conjugated anti-CD13 antibody (1:100 dilution) for 3 h at 4 1C, rinsed the cells, and incubated them for 0-3 h at 37 1C. After being fixed with 4% paraformaldehyde in 0.1 mol/L PBS for 15 min, the cells were permeabilized with 0.1% Triton X-100 for 5 min, treated with 3% bovine serum albumin for 20 min, and labelled for CAV1 through incubating HUVEC with DyLight 488-labelled anti-human CAV1 monoclonal antibody (1:200 dilution) at 4 1C overnight. After being washed for three times in PBS, hoechst33342 staining was performed to stain the nucleus as a control. After extensive washing, the glass bottom dishes were examined under a Zeiss LSM700 laser confocal scanning microscope.
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