Author: Liu, Chunxi; Liu, Tingxian; Yu, Xiaoyue; Gu, Yizhu
Title: A preliminary study on the interaction between Asn-Gly-Arg (NGR)-modified multifunctional nanoparticles and vascular epithelial cells Document date: 2017_4_1
ID: xio0g203_12
Snippet: To observe the localization of CD13, we treated HUVEC cultured in 35-mm glass bottom dishes (Corning Incorporated, USA) with PE-conjugated anti-CD13 antibody (1:100 dilution) for 3 h at 4 1C, rinsed the cells, and incubated them for 0-3 h at 37 1C. After being fixed with 4% paraformaldehyde in 0.1 mol/L PBS for 15 min, the cells were permeabilized with 0.1% Triton X-100 for 5 min, treated with 3% bovine serum albumin for 20 min, and labelled for .....
Document: To observe the localization of CD13, we treated HUVEC cultured in 35-mm glass bottom dishes (Corning Incorporated, USA) with PE-conjugated anti-CD13 antibody (1:100 dilution) for 3 h at 4 1C, rinsed the cells, and incubated them for 0-3 h at 37 1C. After being fixed with 4% paraformaldehyde in 0.1 mol/L PBS for 15 min, the cells were permeabilized with 0.1% Triton X-100 for 5 min, treated with 3% bovine serum albumin for 20 min, and labelled for CAV1 through incubating HUVEC with DyLight 488-labelled anti-human CAV1 monoclonal antibody (1:200 dilution) at 4 1C overnight. After being washed for three times in PBS, hoechst33342 staining was performed to stain the nucleus as a control. After extensive washing, the glass bottom dishes were examined under a Zeiss LSM700 laser confocal scanning microscope.
Search related documents:
Co phrase search for related documents- CAV1 monoclonal antibody and extensive washing: 1, 2
- CAV1 monoclonal antibody and monoclonal antibody: 1, 2, 3
- CAV1 monoclonal antibody and scanning microscope: 1, 2
- confocal scanning microscope and extensive washing: 1, 2, 3
- confocal scanning microscope and laser confocal scanning microscope: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36
- confocal scanning microscope and monoclonal antibody: 1, 2, 3, 4, 5
- confocal scanning microscope and PBS time: 1
- confocal scanning microscope and scanning microscope: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40
- extensive washing and laser confocal scanning microscope: 1
- extensive washing and monoclonal antibody: 1, 2, 3
- extensive washing and scanning microscope: 1, 2, 3
- laser confocal scanning microscope and monoclonal antibody: 1, 2, 3
- laser confocal scanning microscope and PBS time: 1
- laser confocal scanning microscope and scanning microscope: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36
- monoclonal antibody and nucleus stain: 1
- monoclonal antibody and PBS time: 1
- monoclonal antibody and PE conjugate: 1
- monoclonal antibody and scanning microscope: 1, 2, 3, 4, 5, 6
- PBS time and scanning microscope: 1
Co phrase search for related documents, hyperlinks ordered by date