Selected article for: "reverse genetic and wild type"
Author: Agnihothram, Sudhakar; Yount, Boyd L.; Donaldson, Eric F.; Huynh, Jeremy; Menachery, Vineet D.; Gralinski, Lisa E.; Graham, Rachel L.; Becker, Michelle M.; Tomar, Sakshi; Scobey, Trevor D.; Osswald, Heather L.; Whitmore, Alan; Gopal, Robin; Ghosh, Arun K.; Mesecar, Andrew; Zambon, Maria; Heise, Mark; Denison, Mark R.; Baric, Ralph S.
Title: A Mouse Model for Betacoronavirus Subgroup 2c Using a Bat Coronavirus Strain HKU5 Variant
  • Document date: 2014_3_25
    • ID: y4zoyqua_30
    Snippet: The development of infectious clones and recombinant viruses from emerging zoonotic CoVs will expedite the rapid testing of antivirals and vaccines in an outbreak situation. A limitation of the current approach is that wild-type MERS-CoV and BtCoV HKU5 do not replicate in mice. The recent demonstration of the MERS-CoV S glycoprotein bound to its receptor may inspire strategies for designing recombinant MERS-CoV strains that replicate well in mice.....
    Document: The development of infectious clones and recombinant viruses from emerging zoonotic CoVs will expedite the rapid testing of antivirals and vaccines in an outbreak situation. A limitation of the current approach is that wild-type MERS-CoV and BtCoV HKU5 do not replicate in mice. The recent demonstration of the MERS-CoV S glycoprotein bound to its receptor may inspire strategies for designing recombinant MERS-CoV strains that replicate well in mice (54) . Using BtCoV HKU5-SE, we illustrate the utility of synthetic genomics and reverse genetic strategies for resurrecting emerging zoonotic viruses in vitro and validate our findings using drugs and vaccines that can be readily applied in outbreak situations.

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