Title: A Golgi retention signal in a membrane-spanning domain of coronavirus E1 protein Document date: 1991_10_1
ID: s4a8zs5a_9
Snippet: For most of the mutations, the Kunkel method of oligonucleotide-directed mutagenesis (21) was used . The El, Am2,3, and Gml genes were cloned into the M13 vector mp8, and single strands produced in Escherichia coli RZ1032 (dut, ung) . The exception was production of the chimera Gml, which was produced by domain replacement using the oligonucleotide.....
Document: For most of the mutations, the Kunkel method of oligonucleotide-directed mutagenesis (21) was used . The El, Am2,3, and Gml genes were cloned into the M13 vector mp8, and single strands produced in Escherichia coli RZ1032 (dut, ung) . The exception was production of the chimera Gml, which was produced by domain replacement using the oligonucleotide
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