Selected article for: "dna polymerase and Klenow fragment"
Title: A Golgi retention signal in a membrane-spanning domain of coronavirus E1 protein
  • Document date: 1991_10_1
    • ID: s4a8zs5a_13
    Snippet: The membrane-spanning domain of am was replaced with either the IBV El ml or m3 domain using restriction sites in the coding sequence . To create am1G, a BamHI to Rsal fragment (encoding the a subunit) was filled in with the Klenow fragment of DNA polymerase I, digested with Xhol, and ligated with a HpalI to BamHI fragment (encoding the ml domain and G tail) from Gml which was prepared similarly. To create am3G, the same a-encoding fragment descr.....
    Document: The membrane-spanning domain of am was replaced with either the IBV El ml or m3 domain using restriction sites in the coding sequence . To create am1G, a BamHI to Rsal fragment (encoding the a subunit) was filled in with the Klenow fragment of DNA polymerase I, digested with Xhol, and ligated with a HpalI to BamHI fragment (encoding the ml domain and G tail) from Gml which was prepared similarly. To create am3G, the same a-encoding fragment described above was ligated with a Dral to BamHI fragment from Aml,2 (encoding the m3 domain) and a BamHI to XhoI fragment (encoding the G tail) from the G mutant TMB (which has a BamHI site introduced at nucleotide 1483 ; reference 36) .

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