Author: Liu, Chunxi; Liu, Tingxian; Yu, Xiaoyue; Gu, Yizhu
Title: A preliminary study on the interaction between Asn-Gly-Arg (NGR)-modified multifunctional nanoparticles and vascular epithelial cells Document date: 2017_4_1
ID: xio0g203_29
Snippet: To study the effect of endocytic inhibitors on the uptake of TPIC, HUVEC were pre-incubated individually with the following inhibitors at concentrations that were not toxic to the cells 33 : (1) 10 μg/mL of chlorpromazine for 30 min, (2) 1 μg/mL of genistein for 30 min, (3) 30 mmol/L of cytochalasin D for 30 min. Following the pre-incubation, the cells were further treated with freshly prepared TPIC for 4 h. Subsequently, the cells were washed .....
Document: To study the effect of endocytic inhibitors on the uptake of TPIC, HUVEC were pre-incubated individually with the following inhibitors at concentrations that were not toxic to the cells 33 : (1) 10 μg/mL of chlorpromazine for 30 min, (2) 1 μg/mL of genistein for 30 min, (3) 30 mmol/L of cytochalasin D for 30 min. Following the pre-incubation, the cells were further treated with freshly prepared TPIC for 4 h. Subsequently, the cells were washed for three times with PBS, collected according to the methods described above and analyzed by FACS and fluorescence microscopy. In the study, the group without any treatment was used as the background in the FACS analysis, whereas the groups in the presence of TPIC but without inhibitor treatment were used as 100% controls.
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