Author: Liu, Chunxi; Liu, Tingxian; Yu, Xiaoyue; Gu, Yizhu
Title: A preliminary study on the interaction between Asn-Gly-Arg (NGR)-modified multifunctional nanoparticles and vascular epithelial cells Document date: 2017_4_1
ID: xio0g203_27
Snippet: To evaluate the endocytosis kinetics of the TPIC in HUVEC, a transfection dose of TPIC was incubated with HUVEC at 4 1C for 0, 30 min, 1, 3 and 5 h, and at 37 1C for 0, 5, 15, 30 min, 1, 3 and 5 h. After incubation, the culture media were removed and the cells were washed for three times with cold PBS. Then, the cells were labelled with a PE-conjugated anti-CD13 antibody (1:100 dilution) for 3 h at 4 1C. Finally, cells were washed twice with cold.....
Document: To evaluate the endocytosis kinetics of the TPIC in HUVEC, a transfection dose of TPIC was incubated with HUVEC at 4 1C for 0, 30 min, 1, 3 and 5 h, and at 37 1C for 0, 5, 15, 30 min, 1, 3 and 5 h. After incubation, the culture media were removed and the cells were washed for three times with cold PBS. Then, the cells were labelled with a PE-conjugated anti-CD13 antibody (1:100 dilution) for 3 h at 4 1C. Finally, cells were washed twice with cold PBS. The TPIC uptake and surface accessible CD13 after HUVEC incubation with TPIC at 4 or 37 1C for various time periods were determined by FACS.
Search related documents:
Co phrase search for related documents- endocytosis kinetic and TPIC uptake: 1, 2
Co phrase search for related documents, hyperlinks ordered by date