Author: Chen, Yixin; Chan, Kwok-Hung; Kang, Yahong; Chen, Honglin; Luk, Hayes KH; Poon, Rosana WS; Chan, Jasper FW; Yuen, Kwok-Yung; Xia, Ningshao; Lau, Susanna KP; Woo, Patrick CY
Title: A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus Document date: 2015_4_22
ID: puv7kg4k_2
Snippet: Although MERS-CoV can be cultured and antibody detection methods are available, laboratory diagnosis of human MERS-CoV infections is mainly achieved by quantitative real-time RT-PCR, using the RNAdependent RNA polymerase, the region upstream to the envelope gene or the nucleocapsid gene of MERS-CoV as the target. [10] [11] [12] [13] However, the cost of quantitative real-time RT-PCR is still high and such expertise may not be available, particula.....
Document: Although MERS-CoV can be cultured and antibody detection methods are available, laboratory diagnosis of human MERS-CoV infections is mainly achieved by quantitative real-time RT-PCR, using the RNAdependent RNA polymerase, the region upstream to the envelope gene or the nucleocapsid gene of MERS-CoV as the target. [10] [11] [12] [13] However, the cost of quantitative real-time RT-PCR is still high and such expertise may not be available, particularly in the clinical microbiology laboratories in the Middle East where most of the cases were identified. Enzyme-linked immunosorbent assay (ELISA) for antigen detection offers an inexpensive and user-friendly way for laboratory diagnosis of respiratory viral infections, including SARS. 14, 15 As for MERS, antigen detection ELISA will be useful not only for laboratory diagnosis of patients with suspected MERS but also for field studies on dromedaries as well as other suspected animal reservoirs. So far, such an antigen detection assay is not available for MERS-CoV. In this article, we report the development and evaluation of a monoclonal antibody (MAb)-based capture ELISA for MERS-CoV nucleocapsid protein (NP) detection.
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