Author: Liu, Chunxi; Liu, Tingxian; Yu, Xiaoyue; Gu, Yizhu
Title: A preliminary study on the interaction between Asn-Gly-Arg (NGR)-modified multifunctional nanoparticles and vascular epithelial cells Document date: 2017_4_1
ID: xio0g203_41
Snippet: In this section, we tried to examine the interaction between TPIC and CD13. In our previous study, it has been already indicated that NGRmediated nanocarriers entry into HUVEC was an energy dependent active process 33 . In the present study, the uptake was significantly inhibited at 4 1C and TPIC mainly stayed at cell surface but not entered cells. At 4 1C, although TPIC was scattered on the cell membrane as a few green spots, and co-localized wi.....
Document: In this section, we tried to examine the interaction between TPIC and CD13. In our previous study, it has been already indicated that NGRmediated nanocarriers entry into HUVEC was an energy dependent active process 33 . In the present study, the uptake was significantly inhibited at 4 1C and TPIC mainly stayed at cell surface but not entered cells. At 4 1C, although TPIC was scattered on the cell membrane as a few green spots, and co-localized with red CD13 to yellow (Fig. 5A) , indicating that TPIC could bind to CD13 on the cell surface. While, at 37 1C, more green fluorescence was observed Figure 3 Immunofluorescence microscopy (1000 Â ). HUVEC were treated with a PE-labelled mouse anti-human CD13 antibody at 4 1C firstly and incubated at 37 1C for 0, 10, 30, 60 min, 2 or 3 h. White arrows indicated the co-localization of CD13 and CAV1.
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