A preliminary study on the interaction between Asn-Gly-Arg (NGR)-modified multifunctional nanoparticles and vascular epithelial cells

Liu, Chunxi; Liu, Tingxian; Yu, Xiaoyue; Gu, Yizhu

Using an anti-CD13 antibody and anti-CAV1 antibody to label CD13 and CAV1 on HUVEC, respectively, the results of CD13 and CAV1 expression on HUVEC are shown in Fig. 2 . A flow cytometric scatter plot was used for the quantitative analysis. Fig. 2B and C present the individual control of PE anti-human CD13 labelled and DyLight 488 anti-human CAV1 antibody labelled cells, indicating the availability of both antibodies. Fig. 2D shows that the cells are both CD13 and CAV1 positive. Those cells expressing CD13 could be labelled by anti-CD13 antibody and exhibited in red fluorescence (FL2-H, PE, upper left corner) and those CAV1 positive could be labelled by anti-CAV1 antibody and exhibited in green fluorescence (FL1-H, FAM, lower right corner). And those cells expressing both CD13 and CAV1 were showed in upper right corner. The quantitative data demonstrated that the positive percentage of CD13 and CAV1 was 97.75% and 31.94%, respectively, and the percentage of HUVEC expressing both CD13 and CAV1 was 17.07%.