Author: Ranjbar, Shahin; Haridas, Viraga; Jasenosky, Luke D.; Falvo, James V.; Goldfeld, Anne E.
Title: A Role for IFITM Proteins in Restriction of Mycobacterium tuberculosis Infection Document date: 2015_10_22
ID: zy82bo7q_29
Snippet: The MTb strains H37Rv, H37Rv-mCherry (kindly provided by Dr. Sarah Fortune; Sillé et al., 2011) , HN878, and CDC1551 were prepared as described before (Ranjbar et al., 2009) . Cultures were grown to an OD 650 of 0.4 at 37°C to ensure they were logarithmic growth phase, and bacteria were pelleted, washed, resuspended in PBS, and passed through a 5-mM filter to ensure single-cell suspension. Bacterial numbers were determined by measurement of OD .....
Document: The MTb strains H37Rv, H37Rv-mCherry (kindly provided by Dr. Sarah Fortune; Sillé et al., 2011) , HN878, and CDC1551 were prepared as described before (Ranjbar et al., 2009) . Cultures were grown to an OD 650 of 0.4 at 37°C to ensure they were logarithmic growth phase, and bacteria were pelleted, washed, resuspended in PBS, and passed through a 5-mM filter to ensure single-cell suspension. Bacterial numbers were determined by measurement of OD 650 . All the MTb infections were done at a ratio of 1:1 (cells:bacilli).
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