Author: Chen, Yixin; Chan, Kwok-Hung; Kang, Yahong; Chen, Honglin; Luk, Hayes KH; Poon, Rosana WS; Chan, Jasper FW; Yuen, Kwok-Yung; Xia, Ningshao; Lau, Susanna KP; Woo, Patrick CY
Title: A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus Document date: 2015_4_22
ID: puv7kg4k_15
Snippet: A total of 24 MAbs were generated against MERS-CoV-rNP by ELISA. Twelve of them were confirmed to be MERS-CoV-rNP-specific MAbs by ELISA using MERS-CoV-rNP as well as HCoV-229E-rNP and HCoV-OC43-rNP (Figure 3 ). The cloning, expression, and purification procedures for HCoV-229E-rNP and HCoV-OC43-rNP were the same as described for those of MERS-CoV-rNP (data not shown). The other 12 MAbs showed different degrees of cross-reactivity among the three.....
Document: A total of 24 MAbs were generated against MERS-CoV-rNP by ELISA. Twelve of them were confirmed to be MERS-CoV-rNP-specific MAbs by ELISA using MERS-CoV-rNP as well as HCoV-229E-rNP and HCoV-OC43-rNP (Figure 3 ). The cloning, expression, and purification procedures for HCoV-229E-rNP and HCoV-OC43-rNP were the same as described for those of MERS-CoV-rNP (data not shown). The other 12 MAbs showed different degrees of cross-reactivity among the three rNP proteins, suggesting that the NPs of different CoVs might have some common epitopes.
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