Author: Jobling, Michael G.; Yang, ZhiJie; Kam, Wendy R.; Lencer, Wayne I.; Holmes, Randall K.
Title: A Single Native Ganglioside GM(1)-Binding Site Is Sufficient for Cholera Toxin To Bind to Cells and Complete the Intoxication Pathway Document date: 2012_10_30
ID: sxdstw4a_11
Snippet: As an initial step toward investigating whether CT variants with five and one native GM 1 BS trafficked from the cell surface to the ER by the same pathway, we examined the effects of brefeldin A (BFA) on their toxicity for T84 cell monolayers. We found that BFA completely inhibited the I sc (Fig. 5) induced by both single and five native GM 1 BS toxin variants. BFA-treated cells, however, still responded to addition of the cAMP agonist vasoactiv.....
Document: As an initial step toward investigating whether CT variants with five and one native GM 1 BS trafficked from the cell surface to the ER by the same pathway, we examined the effects of brefeldin A (BFA) on their toxicity for T84 cell monolayers. We found that BFA completely inhibited the I sc (Fig. 5) induced by both single and five native GM 1 BS toxin variants. BFA-treated cells, however, still responded to addition of the cAMP agonist vasoactive intestinal polypeptide (VIP) at 90 min, showing that the toxin-treated cells retained viability and were competent for cAMP-dependent Clsecretion (I sc ). Thus, a toxin variant with a single native GM 1 BS, like native CT holotoxin, traffics through a BFA-sensitive pathway to exert its toxic effects.
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