Author: Whitehouse, Caroline; Burchell, Joy; Gschmeissner, Stephen; Brockhausen, Inka; Lloyd, Kenneth O.; Taylor-Papadimitriou, Joyce
Title: A Transfected Sialyltransferase That Is Elevated in Breast Cancer and Localizes to the medial/trans-Golgi Apparatus Inhibits the Development of core-2–based O-Glycans Document date: 1997_6_16
ID: pwgj97kz_23
Snippet: Detection of the Tagged Sialyltransferase in Permeabilized Cells. Cells were stained with 9E10 mAb after incubating the cells with 0.3% saponin for 20 min. Subsequent steps were as previously described (Burchell and Taylor-Papadimitriou, 1993) with the exception that 0.1% saponin was included in all incubations and washes, which were carried out at room temperature. The reactivity of an mAb (LE61) to keratin 18 (Lane, 1982) was included as a posi.....
Document: Detection of the Tagged Sialyltransferase in Permeabilized Cells. Cells were stained with 9E10 mAb after incubating the cells with 0.3% saponin for 20 min. Subsequent steps were as previously described (Burchell and Taylor-Papadimitriou, 1993) with the exception that 0.1% saponin was included in all incubations and washes, which were carried out at room temperature. The reactivity of an mAb (LE61) to keratin 18 (Lane, 1982) was included as a positive control for staining the permeabilized cells.
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