Selected article for: "expression level and wild type"
Author: Whitehouse, Caroline; Burchell, Joy; Gschmeissner, Stephen; Brockhausen, Inka; Lloyd, Kenneth O.; Taylor-Papadimitriou, Joyce
Title: A Transfected Sialyltransferase That Is Elevated in Breast Cancer and Localizes to the medial/trans-Golgi Apparatus Inhibits the Development of core-2–based O-Glycans
  • Document date: 1997_6_16
    • ID: pwgj97kz_37
    Snippet: Northern blot analysis revealed that all the selected cell lines were expressing the transfected DNA (Fig. 2 a) and, moreover, the expression level varied with the clone, with the MTSV1-7 transfectants expressing more than the T47D clones. The difference in molecular weight between the message in T47D clones and the MTSV1-7 is due to the ␣2,3 sialyltransferase RNA in the MTSV1-7 cells being expressed within the context of the retrovirus vector......
    Document: Northern blot analysis revealed that all the selected cell lines were expressing the transfected DNA (Fig. 2 a) and, moreover, the expression level varied with the clone, with the MTSV1-7 transfectants expressing more than the T47D clones. The difference in molecular weight between the message in T47D clones and the MTSV1-7 is due to the ␣2,3 sialyltransferase RNA in the MTSV1-7 cells being expressed within the context of the retrovirus vector. Fig. 2 b shows endogenous expression of ␣2,3 SAT (O) in T47D and MTSV1-7 parental cell lines, where the Northern blot was exposed for a longer period of time. wild-type T47D and MTSV1-7 cells. Blots were prepared and probed as described in Materials and Methods. The bands representing the transfected gene are shown in a after overnight exposure of the film. The difference in size of the mRNA transcript in T47D and MTSV1-7 transfectants is due to the use of different vectors for introducing the gene. The blot in b was exposed for 6 d to detect endogenous transcripts.

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