Selected article for: "HUVEC TPIC internalization and TPIC internalization"

Author: Liu, Chunxi; Liu, Tingxian; Yu, Xiaoyue; Gu, Yizhu
Title: A preliminary study on the interaction between Asn-Gly-Arg (NGR)-modified multifunctional nanoparticles and vascular epithelial cells
  • Document date: 2017_4_1
  • ID: xio0g203_21
    Snippet: To determine whether the CD13 activity affected the internalization of TPIC, HUVEC was pre-treated with 100 μg/mL bestatin 35 , a CD13/APN inhibitor, for 1 h at room temperature in PBS. Then, cells were washed for three times with PBS. TPIC were added into wells and incubated with cells for another 4 h at 37 1C with 5% CO 2 in an incubator. Finally, all cells were harvested and washed, and the cell-associated fluorescence was determined by FACS......
    Document: To determine whether the CD13 activity affected the internalization of TPIC, HUVEC was pre-treated with 100 μg/mL bestatin 35 , a CD13/APN inhibitor, for 1 h at room temperature in PBS. Then, cells were washed for three times with PBS. TPIC were added into wells and incubated with cells for another 4 h at 37 1C with 5% CO 2 in an incubator. Finally, all cells were harvested and washed, and the cell-associated fluorescence was determined by FACS.

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