Author: Ranjbar, Shahin; Haridas, Viraga; Jasenosky, Luke D.; Falvo, James V.; Goldfeld, Anne E.
Title: A Role for IFITM Proteins in Restriction of Mycobacterium tuberculosis Infection Document date: 2015_10_22
ID: zy82bo7q_47
Snippet: THP-1 cells stably expressing IFITM3-V5 tag or empty vector were infected with H37Rv MTb for 48 hr, at which time they were incubated with LysoTracker Red DND-99 (LTRed, Life Technologies) at 50 nM for 2 hr at 37°C. Cells were washed and fixed with 4% paraformaldehyde before analysis by flow cytometry as described above......
Document: THP-1 cells stably expressing IFITM3-V5 tag or empty vector were infected with H37Rv MTb for 48 hr, at which time they were incubated with LysoTracker Red DND-99 (LTRed, Life Technologies) at 50 nM for 2 hr at 37°C. Cells were washed and fixed with 4% paraformaldehyde before analysis by flow cytometry as described above.
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