Selected article for: "cell line and normal cell"
Author: Whitehouse, Caroline; Burchell, Joy; Gschmeissner, Stephen; Brockhausen, Inka; Lloyd, Kenneth O.; Taylor-Papadimitriou, Joyce
Title: A Transfected Sialyltransferase That Is Elevated in Breast Cancer and Localizes to the medial/trans-Golgi Apparatus Inhibits the Development of core-2–based O-Glycans
  • Document date: 1997_6_16
    • ID: pwgj97kz_35
    Snippet: The cell line MTSV1-7 has many characteristics of normal mammary epithelial cells. It is nontumorigenic, forms organized colonies in collagen gels (Berdichevsky and Taylor-Papadimitriou, 1991; Shearer et al., 1992; Lu et al., 1995) , and glycosylates MUC1 in a manner similar to that of normal mammary epithelial cells in adding core-2-based O-glycans (Lloyd et al., 1996) . MTSV1-7 has proven to be difficult to transfect, and a retrovirus expressin.....
    Document: The cell line MTSV1-7 has many characteristics of normal mammary epithelial cells. It is nontumorigenic, forms organized colonies in collagen gels (Berdichevsky and Taylor-Papadimitriou, 1991; Shearer et al., 1992; Lu et al., 1995) , and glycosylates MUC1 in a manner similar to that of normal mammary epithelial cells in adding core-2-based O-glycans (Lloyd et al., 1996) . MTSV1-7 has proven to be difficult to transfect, and a retrovirus expressing the myctagged ␣2,3 sialyltransferase was constructed (see Materials and Methods) and used to transduce MTSV1-7. Five stable cell lines (designated MTSV1-7 3ST 3.10, 3.11, 3.14, 4.1, and 5.2) were selected in the presence of puromycin.

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