Author: Whitehouse, Caroline; Burchell, Joy; Gschmeissner, Stephen; Brockhausen, Inka; Lloyd, Kenneth O.; Taylor-Papadimitriou, Joyce
Title: A Transfected Sialyltransferase That Is Elevated in Breast Cancer and Localizes to the medial/trans-Golgi Apparatus Inhibits the Development of core-2–based O-Glycans Document date: 1997_6_16
ID: pwgj97kz_11
Snippet: T47D cell line was transfected directly with the neo3STMYC construct using the method of calcium phosphate transfection described above with the following alterations. The DNA precipitate was left on the cells overnight before glycerol shocking the cells for 3 min. The cells were then washed three times with PBS before refeeding with fresh medium. 2 d later, the dishes were split 1:10 into medium containing 500 g/ml G418, and selection was carrie.....
Document: T47D cell line was transfected directly with the neo3STMYC construct using the method of calcium phosphate transfection described above with the following alterations. The DNA precipitate was left on the cells overnight before glycerol shocking the cells for 3 min. The cells were then washed three times with PBS before refeeding with fresh medium. 2 d later, the dishes were split 1:10 into medium containing 500 g/ml G418, and selection was carried out until individual clones were isolated and expanded. Selected clones were referred to as T47D 3STMYC. Cells were also transfected with the vector pcDNA1neo and a clone, T47D neo, was isolated.
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