Author: Whitehouse, Caroline; Burchell, Joy; Gschmeissner, Stephen; Brockhausen, Inka; Lloyd, Kenneth O.; Taylor-Papadimitriou, Joyce
Title: A Transfected Sialyltransferase That Is Elevated in Breast Cancer and Localizes to the medial/trans-Golgi Apparatus Inhibits the Development of core-2–based O-Glycans Document date: 1997_6_16
ID: pwgj97kz_12
Snippet: For transduction of MTSV1-7 cells with the ⣠2,3 sialyltransferase retrovirus, the cells were grown to 70% confluency in 10-cm dishes and infected with 3 ml of viral stock (with or without dilution) with 8 g/ml polybrene. Infection proceeded for 3 h before virus was replaced with fresh medium. 2 d later, the plates were split 1:10 into medium containing 2 g/ml puromycin, and selection continued until individual colonies could be identified and .....
Document: For transduction of MTSV1-7 cells with the ⣠2,3 sialyltransferase retrovirus, the cells were grown to 70% confluency in 10-cm dishes and infected with 3 ml of viral stock (with or without dilution) with 8 g/ml polybrene. Infection proceeded for 3 h before virus was replaced with fresh medium. 2 d later, the plates were split 1:10 into medium containing 2 g/ml puromycin, and selection continued until individual colonies could be identified and expanded. Selected clones were referred to as MTSV1-7 3STMYC. Cells were also infected with a retrovirus derived from the pBabe puro vector, and a clone MTSV1-7 pBpuro was isolated.
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