A Role for IFITM Proteins in Restriction of Mycobacterium tuberculosis Infection

Ranjbar, Shahin; Haridas, Viraga; Jasenosky, Luke D.; Falvo, James V.; Goldfeld, Anne E.

The mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems) with the following gene-specific primers: IFITM1 (forward, 5′-ATCCTGTTACTGGTATTCGG-3′; reverse, 5′-TATAAACTGCTGTATCTAGG-3′); IFITM2 (forward, 5′-GTTGGTCGTCCAGGCCCAGC-3′; reverse, 5′-CTGTGGGGACAGGGCGAGGA-3′); IFITM3 (forward, 5′-GCTGATCTTCCAGGCCTATG-3′; reverse, 5′-GATACAGGACTCGGCTCCGG-3); IL1B (forward, 5′-GCTGAGGAAGATGCTGGTTC-3′; reverse, 5′-TCCATATCCTGTCCCTGGAG-3′); IL-6 (forward, 5′-AGGAGACTTGCCTGGTGAAA-3′; reverse, 5′-CAGGGGTGGTTATTGCATCT-3′); IFNB1 (forward, 5′-GAATGGGAGGCTTGAATACTGCCT-3′; reverse, 5′-TAGCAAAGATGTTCTGGAGCATCTC-3′); TNF (forward, 5′-TCTTCTCGAACCCCGAGTGA-3′; reverse, 5′-CCTCTGATGGCACCACCA-3′); and cyclophilin B (PPIB) (forward, 5′-AGAAGAAGGGGCCCAAAG-3′; reverse, 5′-AAAGATCACCCGGCCTACA-3′).