Selected article for: "acid concentration and lysis buffer"
Author: WU, Hong-Xia; WANG, Hua-Lei; GUO, Xiao-Feng; YANG, Yu-Jiao; MA, Jin-Zhu; WANG, Tie-Cheng; GAO, Yu-Wei; ZHAO, Yong-Kun; YANG, Song-Tao; XIA, Xian-Zhu
Title: Adeno-Associated Viruses Serotype 2-Mediated RNA Interference Efficiently Inhibits Rabies Virus Replication In Vitro and In Vivo
  • Document date: 2013_6_14
    • ID: sj9k4c3i_11
    Snippet: Western blotting: NA cells were harvested after 72 hr of RAbV infection, and total protein samples were obtained by incubation in cell lysis buffer (beyotime biotech Inc., Nantong, China). Protein concentration was measured with a bicinchoninic acid (bCA) protein assay (Pierce, Rockford, IL, U.S.A.). Fifty microgram aliquots of total protein were resolved by sodium SDS-PAGE and transferred to a nitrocellulose membrane (Amersham biosciences, Uppsa.....
    Document: Western blotting: NA cells were harvested after 72 hr of RAbV infection, and total protein samples were obtained by incubation in cell lysis buffer (beyotime biotech Inc., Nantong, China). Protein concentration was measured with a bicinchoninic acid (bCA) protein assay (Pierce, Rockford, IL, U.S.A.). Fifty microgram aliquots of total protein were resolved by sodium SDS-PAGE and transferred to a nitrocellulose membrane (Amersham biosciences, Uppsala, Sweden). The resultant blots were probed with a mouse monoclonal antibody to RAbV N protein (1:200) or a mouse monoclonal antibody to β-actin (1:2,000, Santa Cruz Biotechnology, Santa Cruz, CA, U.S.A.), followed by the horseradish peroxidase-conjugated goat anti-mouse IgG (1:2,000, Santa Cruz Biotechnology). Immunoreactive bands were detected with enhanced chemiluminescence reagent (Pierce).

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