Author: WU, Hong-Xia; WANG, Hua-Lei; GUO, Xiao-Feng; YANG, Yu-Jiao; MA, Jin-Zhu; WANG, Tie-Cheng; GAO, Yu-Wei; ZHAO, Yong-Kun; YANG, Song-Tao; XIA, Xian-Zhu
Title: Adeno-Associated Viruses Serotype 2-Mediated RNA Interference Efficiently Inhibits Rabies Virus Replication In Vitro and In Vivo Document date: 2013_6_14
ID: sj9k4c3i_11
Snippet: Western blotting: NA cells were harvested after 72 hr of RAbV infection, and total protein samples were obtained by incubation in cell lysis buffer (beyotime biotech Inc., Nantong, China). Protein concentration was measured with a bicinchoninic acid (bCA) protein assay (Pierce, Rockford, IL, U.S.A.). Fifty microgram aliquots of total protein were resolved by sodium SDS-PAGE and transferred to a nitrocellulose membrane (Amersham biosciences, Uppsa.....
Document: Western blotting: NA cells were harvested after 72 hr of RAbV infection, and total protein samples were obtained by incubation in cell lysis buffer (beyotime biotech Inc., Nantong, China). Protein concentration was measured with a bicinchoninic acid (bCA) protein assay (Pierce, Rockford, IL, U.S.A.). Fifty microgram aliquots of total protein were resolved by sodium SDS-PAGE and transferred to a nitrocellulose membrane (Amersham biosciences, Uppsala, Sweden). The resultant blots were probed with a mouse monoclonal antibody to RAbV N protein (1:200) or a mouse monoclonal antibody to β-actin (1:2,000, Santa Cruz Biotechnology, Santa Cruz, CA, U.S.A.), followed by the horseradish peroxidase-conjugated goat anti-mouse IgG (1:2,000, Santa Cruz Biotechnology). Immunoreactive bands were detected with enhanced chemiluminescence reagent (Pierce).
Search related documents:
Co phrase search for related documents- bCA bicinchoninic acid and chemiluminescence reagent: 1
- bCA bicinchoninic acid and nitrocellulose membrane: 1, 2
- bCA bicinchoninic acid and protein assay: 1, 2, 3, 4, 5, 6, 7
- bCA bicinchoninic acid and protein assay bCA bicinchoninic acid: 1, 2, 3, 4, 5
- bicinchoninic acid and chemiluminescence reagent: 1
- bicinchoninic acid and nitrocellulose membrane: 1, 2, 3
- bicinchoninic acid and protein assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
- bicinchoninic acid and protein assay bCA bicinchoninic acid: 1, 2, 3, 4, 5
- cell lysis and monoclonal antibody: 1, 2
- cell lysis and NA cell: 1
- cell lysis and nitrocellulose membrane: 1, 2
- cell lysis and protein assay: 1, 2, 3, 4, 5
- cell lysis buffer and monoclonal antibody: 1, 2
- cell lysis buffer and nitrocellulose membrane: 1, 2
- cell lysis buffer and protein assay: 1, 2, 3, 4
- chemiluminescence reagent and nitrocellulose membrane: 1, 2
- chemiluminescence reagent and protein assay: 1, 2
- chemiluminescence reagent and protein assay bCA bicinchoninic acid: 1
- monoclonal antibody and mouse monoclonal antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
Co phrase search for related documents, hyperlinks ordered by date