Author: Dai, Zhi; Arévalo, Maria T; Li, Junwei; Zeng, Mingtao
Title: Addition of poly (propylene glycol) to multiblock copolymer to optimize siRNA delivery Document date: 2014_1_1
ID: yawwtyxg_26
Snippet: Neuro 2a/GFP cells (8 × 10 3 cells per well) were seeded in 12-wellplates 48 h before transfection. Medium was replaced with serum-free medium, and cells were treated with 20 μl of different complexes with a final concentration of 80 nM Cy3-labeled siRNA. At 4 h post-transfection, cells were imaged using a Nikon Ti Eclipse fluorescent microscope for GFP expression and uptake of Cy3-labeled siRNA......
Document: Neuro 2a/GFP cells (8 × 10 3 cells per well) were seeded in 12-wellplates 48 h before transfection. Medium was replaced with serum-free medium, and cells were treated with 20 μl of different complexes with a final concentration of 80 nM Cy3-labeled siRNA. At 4 h post-transfection, cells were imaged using a Nikon Ti Eclipse fluorescent microscope for GFP expression and uptake of Cy3-labeled siRNA.
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