Author: Feng, Joy Y
Title: Addressing the selectivity and toxicity of antiviral nucleosides Document date: 2018_3_13
ID: uajl5wyk_10
Snippet: As shown in Table 1 , we employed three mitochondriafocused cell-based assays to evaluate potential mitochondrial toxicity. The mitochondrial DNA content assay has been widely used to study 2 0 -deoxy nucleoside analogs, but it does not assess the effect of ribose nucleoside/tide analogs on mitochrondrial RNA synthesis. 20,29 ddC serves as a good positive control and HepG2 cells seem to be an ideal cell line for this assay. The mitochondrial prot.....
Document: As shown in Table 1 , we employed three mitochondriafocused cell-based assays to evaluate potential mitochondrial toxicity. The mitochondrial DNA content assay has been widely used to study 2 0 -deoxy nucleoside analogs, but it does not assess the effect of ribose nucleoside/tide analogs on mitochrondrial RNA synthesis. 20,29 ddC serves as a good positive control and HepG2 cells seem to be an ideal cell line for this assay. The mitochondrial protein synthesis assay has been useful to evaluate ribonucleoside/tide analogs. In this assay, the levels of two proteins are measured simultaneously: the mitochondrial DNA-encoded protein cytochrome c oxidase 1 (COX-1) and a nuclear DNA-encoded protein succinate dehydrogenase A (SDH-A). 32 For data analysis, we found that comparing the individual levels of the COX-1 and SDH-A proteins to the untreated DMSO control yielded more reproducible data than using the ratio of COX-1:SDH-A. Chloramphenicol, a specific inhibitor of mitochondrial protein synthesis, is used as the positive control.
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