Adeno-Associated Viruses Serotype 2-Mediated RNA Interference Efficiently Inhibits Rabies Virus Replication In Vitro and In Vivo

WU, Hong-Xia; WANG, Hua-Lei; GUO, Xiao-Feng; YANG, Yu-Jiao; MA, Jin-Zhu; WANG, Tie-Cheng; GAO, Yu-Wei; ZHAO, Yong-Kun; YANG, Song-Tao; XIA, Xian-Zhu

To check whether the antiviral activity of the rAAV-N796 in NA cells depended on the inoculation titer of rAAV-N796, as show in Fig. 2C and 2E, the NA cells were transduced with different titers of rAAV-N796 (1, 10 and 100 vg/cell) and were infected with 0.01 MOI of the RAbV CVS-11 virus after transduction for 24 hr. At 72 hr post infection, the cells were collected for assay of N gene mRNA level and protein level. The results showed that the N gene mRNA level was reduced by 78.54 ± 6.8, 45.61 ± 5.3 and 25.10 ± 3.5%, respectively, compared with the rAAV-Neg group. When NA cells were infected with RABV firstly and then transduced with different titers of rAAV-N796 (1, 10 and 100 vg/cell) ( Fig. 2D and 2F ), a significant lower N gene mRNA level (P<0.05) was observed in higher rAAV-N796 titer groups (10 and 100 vg/cell), but not in the lower rAAV-N796 titer group (1 vg/cell). The N protein level also showed the similar result. The higher the rAAV-N796 titer was, the lower the N protein level was, which indicated that the antiviral activity of the rAAV-N796 in NA cells depended on the titer of rAAV-N796.