Author: WU, Hong-Xia; WANG, Hua-Lei; GUO, Xiao-Feng; YANG, Yu-Jiao; MA, Jin-Zhu; WANG, Tie-Cheng; GAO, Yu-Wei; ZHAO, Yong-Kun; YANG, Song-Tao; XIA, Xian-Zhu
Title: Adeno-Associated Viruses Serotype 2-Mediated RNA Interference Efficiently Inhibits Rabies Virus Replication In Vitro and In Vivo Document date: 2013_6_14
ID: sj9k4c3i_7
Snippet: Plasmid construction: pAAV-hrGFP, pAAV-RC and pHelper are described in the information provided for the AAV Helper-Free System (Catalog #240071, Agilent Technologies, Santa Clara, CA, U.S.A.). siRNA-796 targeting the N gene was inserted into the pSilence2.1-U6 Hygro siRNA expression vector (Ambion, Austin, TX, U.S.A.) by bamHI and HindIII (ps-N796). The ps-N796 plasmid transported by a liposome could inhibit RAbV replication in vitro and vivo [20.....
Document: Plasmid construction: pAAV-hrGFP, pAAV-RC and pHelper are described in the information provided for the AAV Helper-Free System (Catalog #240071, Agilent Technologies, Santa Clara, CA, U.S.A.). siRNA-796 targeting the N gene was inserted into the pSilence2.1-U6 Hygro siRNA expression vector (Ambion, Austin, TX, U.S.A.) by bamHI and HindIII (ps-N796). The ps-N796 plasmid transported by a liposome could inhibit RAbV replication in vitro and vivo [20] . The siRNA expressing box containing CMV, cGFP, U6 promoter and siRNA was amplified, and NotI was introduced into 5' and 3'. The siRNA-expressing box was digested with NotI and inserted into pAAV-hrGFP treated with NotI (pAAV-N796). The negative control was named with pAAV-Neg. The pAAV-N796 encoding the specific shRNAs is shown in Fig. 1 .
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