Author: WU, Hong-Xia; WANG, Hua-Lei; GUO, Xiao-Feng; YANG, Yu-Jiao; MA, Jin-Zhu; WANG, Tie-Cheng; GAO, Yu-Wei; ZHAO, Yong-Kun; YANG, Song-Tao; XIA, Xian-Zhu
Title: Adeno-Associated Viruses Serotype 2-Mediated RNA Interference Efficiently Inhibits Rabies Virus Replication In Vitro and In Vivo Document date: 2013_6_14
ID: sj9k4c3i_9
Snippet: Viral titer assay: Viral titer was measured using 50% tissue culture infective dose (TCID 50 ) assays. Serial 10-fold dilutions of supernatants from the treated and viral samples were added onto a monolayer of bHK-21 cells in 96-well culture plates and incubated for two days. Virus concentrations were measured by direct fluorescent antibody staining (DFA), as described above, and the viral titer for each sample was calculated by the Reed-Muench m.....
Document: Viral titer assay: Viral titer was measured using 50% tissue culture infective dose (TCID 50 ) assays. Serial 10-fold dilutions of supernatants from the treated and viral samples were added onto a monolayer of bHK-21 cells in 96-well culture plates and incubated for two days. Virus concentrations were measured by direct fluorescent antibody staining (DFA), as described above, and the viral titer for each sample was calculated by the Reed-Muench method [15] .
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