Author: Crameri, Gary; Durr, Peter A.; Barr, Jennifer; Yu, Meng; Graham, Kerryne; Williams, Owen J.; Kayali, Ghazi; Smith, David; Peiris, Malik; Mackenzie, John S.; Wang, Lin-Fa
Title: Absence of MERS-CoV antibodies in feral camels in Australia: Implications for the pathogen's origin and spread Document date: 2015_11_2
ID: yxtepbta_11
Snippet: VNT was conducted as previously described for SARS-CoV [10, 25] . Briefly, each camel serum was tested in duplicate by doubling dilution in EMEM starting at 1:10 out to 1:1280. To 50 μl of sera an equal volume of EMEM containing 200 TCID50 of a Dromedary camel isolate of MERS virus [24] was added and incubated for 30 min at 37°C. Vero cells were then added to each well and the plates incubated at 37°C and subsequently read for the presence of .....
Document: VNT was conducted as previously described for SARS-CoV [10, 25] . Briefly, each camel serum was tested in duplicate by doubling dilution in EMEM starting at 1:10 out to 1:1280. To 50 μl of sera an equal volume of EMEM containing 200 TCID50 of a Dromedary camel isolate of MERS virus [24] was added and incubated for 30 min at 37°C. Vero cells were then added to each well and the plates incubated at 37°C and subsequently read for the presence of cytopathic effect (CPE) after 4 days. Neutralising titres were recorded at the dilution at which at least one duplicate well was negative for CPE. The same positive control camel sera were used in this assay.
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