Title: Age-related factors in cyclosporine-induced syngeneic graft-versus-host disease: regulatory role of marrow-derived T lymphocytes Document date: 1990_7_1
ID: wmjjoyso_15
Snippet: Flow MicroJiuorimetry Analysis. After each type of cell separation listed above, the depleted populations were stained with an FITC conjugate o£ the primary antibody used for depletion, an FITC conjugate of the mAb to the reciprocal subset, or an isotypespecific FITC sheep anti-mouse IgG to detect residual cells coated with the primary antibody used in selection. The single immunofluorescence was analyzed on a FACScan analytical scanner (Hewlett.....
Document: Flow MicroJiuorimetry Analysis. After each type of cell separation listed above, the depleted populations were stained with an FITC conjugate o£ the primary antibody used for depletion, an FITC conjugate of the mAb to the reciprocal subset, or an isotypespecific FITC sheep anti-mouse IgG to detect residual cells coated with the primary antibody used in selection. The single immunofluorescence was analyzed on a FACScan analytical scanner (Hewlett-Packard Co., No Alto, CA). The negatively selected populations were less than 5% contaminated by the depleted cell subset on average.
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