Selected article for: "dna virus and virus replication"
Author: WU, Hong-Xia; WANG, Hua-Lei; GUO, Xiao-Feng; YANG, Yu-Jiao; MA, Jin-Zhu; WANG, Tie-Cheng; GAO, Yu-Wei; ZHAO, Yong-Kun; YANG, Song-Tao; XIA, Xian-Zhu
Title: Adeno-Associated Viruses Serotype 2-Mediated RNA Interference Efficiently Inhibits Rabies Virus Replication In Vitro and In Vivo
  • Document date: 2013_6_14
    • ID: sj9k4c3i_26
    Snippet: The AAV-2 genome is a linear, single-stranded DNA of 4.7 kb [1] . After infection, second-strand synthesis is needed. So, the gene expression time would be later than other double-stranded DNA virus vectors. In the in vitro experiment, if rAAV-N796 treatment was later than RAbV infection, the antiviral effect was not displayed at 24 and 48 hr post infection, but was displayed at 72 and 96 hr post infection (Fig. 2b) . The reason for this may be a.....
    Document: The AAV-2 genome is a linear, single-stranded DNA of 4.7 kb [1] . After infection, second-strand synthesis is needed. So, the gene expression time would be later than other double-stranded DNA virus vectors. In the in vitro experiment, if rAAV-N796 treatment was later than RAbV infection, the antiviral effect was not displayed at 24 and 48 hr post infection, but was displayed at 72 and 96 hr post infection (Fig. 2b) . The reason for this may be ascribe to the later gene expression. After the AAV-2 synthesizes the second-strand, shRNA begins to be transcribed by the U6 promoter. During the time of second-strand synthesis, the replication of RAbV was not inhibited, and the virus has replicated for several cycles. The RAbV titers of rAAV-N796 and rAAV-Neg treatment groups were both increased with the same efficiency. So, the antiviral effect was not observed in early infection.

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