Author: Zirkel, Florian; Kurth, Andreas; Quan, Phenix-Lan; Briese, Thomas; Ellerbrok, Heinz; Pauli, Georg; Leendertz, Fabian H.; Lipkin, W. Ian; Ziebuhr, John; Drosten, Christian; Junglen, Sandra
Title: An Insect Nidovirus Emerging from a Primary Tropical Rainforest Document date: 2011_6_14
ID: ulwo6i38_23
Snippet: Virus isolation and purification. Virus isolation from 432 pools of 4,839 female mosquito heads was done with Aedes albopictus (C6/36) cells as described previously (15) . For virus growth kinetics, C6/36 cells were infected with an MOI of 0.1, 0.01, or 0.001 and incubated for 1 h at 28°C. The inoculum was removed, and cells were washed with phosphatebuffered saline (PBS). L-15 medium was added, and cells were incubated for 48 h. Every 3 h, an a.....
Document: Virus isolation and purification. Virus isolation from 432 pools of 4,839 female mosquito heads was done with Aedes albopictus (C6/36) cells as described previously (15) . For virus growth kinetics, C6/36 cells were infected with an MOI of 0.1, 0.01, or 0.001 and incubated for 1 h at 28°C. The inoculum was removed, and cells were washed with phosphatebuffered saline (PBS). L-15 medium was added, and cells were incubated for 48 h. Every 3 h, an aliquot of the cell culture supernatant was removed, RNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany), cDNA was synthesized using the SuperScript III RT System (Invitrogen, Karlsruhe, Germany), and CAVV viral genome copy numbers were quantified by real-time RT-PCR (14) .
Search related documents:
Co phrase search for related documents- Aedes albopictus and real time: 1, 2
- cell culture and genome copy: 1, 2, 3, 4, 5
- cell culture and genome copy number: 1, 2
- cell culture and Mini kit: 1, 2, 3, 4, 5, 6, 7
- cell culture and previously describe: 1
- cell culture and QIAamp viral RNA Mini kit: 1, 2, 3, 4
- cell culture supernatant and genome copy: 1
- cell culture supernatant and genome copy number: 1
- cell culture supernatant and Mini kit: 1, 2, 3
- cell culture supernatant and previously describe: 1
- cell culture supernatant and QIAamp viral RNA Mini kit: 1, 2, 3
- cell MOI infect and MOI infect: 1
- genome copy and Mini kit: 1
- Mini kit and QIAamp viral RNA Mini kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
Co phrase search for related documents, hyperlinks ordered by date