Selected article for: "cell virus and real time"
Author: Zirkel, Florian; Kurth, Andreas; Quan, Phenix-Lan; Briese, Thomas; Ellerbrok, Heinz; Pauli, Georg; Leendertz, Fabian H.; Lipkin, W. Ian; Ziebuhr, John; Drosten, Christian; Junglen, Sandra
Title: An Insect Nidovirus Emerging from a Primary Tropical Rainforest
  • Document date: 2011_6_14
    • ID: ulwo6i38_24
    Snippet: To obtain pure virus stocks, CAVV was titrated on insect cells and cell culture supernatant was harvested at 22 hpi to allow only single-round infections. Numbers of genome copies were measured by real-time RT-PCR, and the infectious supernatant of the highest dilution still showing virus replication was used for retitration of CAVV on insect cells. This procedure was repeated five times......
    Document: To obtain pure virus stocks, CAVV was titrated on insect cells and cell culture supernatant was harvested at 22 hpi to allow only single-round infections. Numbers of genome copies were measured by real-time RT-PCR, and the infectious supernatant of the highest dilution still showing virus replication was used for retitration of CAVV on insect cells. This procedure was repeated five times.

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