Selected article for: "cell culture and Electron microscopy"

Author: Zirkel, Florian; Kurth, Andreas; Quan, Phenix-Lan; Briese, Thomas; Ellerbrok, Heinz; Pauli, Georg; Leendertz, Fabian H.; Lipkin, W. Ian; Ziebuhr, John; Drosten, Christian; Junglen, Sandra
Title: An Insect Nidovirus Emerging from a Primary Tropical Rainforest
  • Document date: 2011_6_14
  • ID: ulwo6i38_5
    Snippet: Prevalence and divergence of CAVV along a gradient of habitat modifications. Between February and June 2004, 7,067 mosquitoes were trapped along an anthropogenic disturbance gradient in the area of the Ta National Park, Côte d'Ivoire. The gradient comprised sampling sites in the primary (pristine) forest, in secondary (modified) forest, in agriculturally exploited forest edge areas, and in adjacent human settlements (14) (15) (16) . Initial anal.....
    Document: Prevalence and divergence of CAVV along a gradient of habitat modifications. Between February and June 2004, 7,067 mosquitoes were trapped along an anthropogenic disturbance gradient in the area of the Ta National Park, Côte d'Ivoire. The gradient comprised sampling sites in the primary (pristine) forest, in secondary (modified) forest, in agriculturally exploited forest edge areas, and in adjacent human settlements (14) (15) (16) . Initial analyses by cell culture and electron microscopy yielded viral particles with CoV-like morphology in supernatants of C6/36 cells infected with mosquito homogenates. A short genome fragment with low but significant identity to ORF1b of CoV was identified (14) . This virus was tentatively named Cavally virus (CAVV), after a river near the sites where mosquitoes were caught.

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